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Fluorescent Peptide
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Product: Views:229Fluorescent Peptide 
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Valid until: Long-term effective
Last updated: 2024-08-04 20:22
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Fluorescence and Dye labeling peptides

 

Fluorescence and dye labeled peptides have been developed for in vivo biomedical imaging, protein binding and localization studies, etc. Fluorescence and dye can be attached to the peptide N-terminus directly (FITC always via be inserted an aminohexanoic acid (Ahx) or β-Ala linker between the fluorophore (fluoroscein) and the N-terminus of the peptide.), or to the Lys (or Cys) side chains in the peptide C-terminus or many other positions in the peptide.

 

Biorunstar can provide many common used fluorescent and dyes peptides: such as FITC, FAM, TAMRA, NBD (7-nitrobenz-2-oxa-1, 3-diazole), Amc, Atto, Alexa Fluor, Bodipy, Cy3, Cy5......

 

Each fluorescence and dye labeled peptide delivered as a lyophilized powder in foil covered tube.
 

FITC

Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye has been extensively used to label peptides and proteins.

 

Labeled on the peptide N-terminus: Labeled on the lysine side chain:

 

Fluorescein or dye

Color

Absorption maximum

Emission maximum

FITC

Green

495nm

521nm

 

FAM

FAM is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

 

Rhodamine B

Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescence assays.

 

Fluorescein or dye

Color

Absorption maximum

Emission maximum

Rhodamine B

Orange

550 nm

580 nm

 

TAMRA

TAMRA is one of the most popular orange fluorophores used to label peptides and proteins.

 

Fluorescein or dye

Color

Absorption maximum

Emission maximum

Rhodamine B

Orange

544nm

576 nm

 

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD-labeled peptides is reported as a popular choice in membrane and cell biological studies.

 

Fluorescein or dye

Color

Absorption maximum

Emission maximum

NBD

orange

486 nm

543 nm

 

AMC

AMC (7-amino-4-methylcoumarinyl), UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

 

Fluorescein or dye

Color

Absorption maximum

Emission maximum

AMC

blue

353 nm

422 nm

 

Cy3 and Cy5

Cy3 and Cy5 are dyes with extremely high extinction coefficients and fluorescence. Thus, they are especially suitable for very sensitive localisation assays of peptides in cells.Their disadvantage is the high instability of the molecules under peptide synthesis conditions.

Therefore the yields are comparatively low.

 

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