Shenzhen Biorunstar Biotechnology Co., Ltd

 

Biorunstar is a full-service peptide company. We utilize solid-phase peptide synthesis, solution-phase synthesis, and microwave synthesis chemistry to prepare your custom peptide product. We purify the desired peak using preparative HPLC, and the final peptide sample is reanalyzed with reverse-phase HPLC to verify purity. Mass spectrometry is used to confirm the peptide's mass, and HNMR analysis reports are provided to verify special small compound-peptide complexes.

 

Overview of peptide synthesis service range scope

Length of sequence from 2 to 120 amino acids
Peptide amounts from mg -kg
Different levels of purity, crude, desalt, >70, >80, > 90, >95% and >98%
Cyclic Peptides - Cys to Cys, Head to Tail, Internal Lactam
Simple and complex peptide modifications
Monthly supplied capacity over 1000 peptides
Aliquots of appropriate sizes as customer requested
Most peptide orders can be delivered within 2~3 weeks. The lead time generally vary depens on the peptide length, quantity and difficulty.
Every peptide are supplied with stringent analytical specifications, which includes HPLC chromatograms to ensure purity and mass spectral analysis to confirm identity.

 

Peptide synthesis modified at C terminus

 

Peptide synthesis modified at N terminus

 

Peptide Amidation and Peptide Acetylation

If the peptide originates from an internal protein sequence, In the protein, the peptide fragment's ends would be amides (CONH2), so amidating the C-terminus and acetylating the N-terminus mimics this natural state. These modifications eliminate the peptide's charge and aid in protecting it from enzymatic degradation.

 

Peptide Amidation:	Peptide Acetylation:

Aldehyde peptide: peptide aldehydes have the inhibition properties towards various enzymes. Furthermore, peptide aldehydes are of great interest for peptide backbone modification or ligation reactions.

 

CMK/FMK labeled peptide: Peptide chloromethylketones (CMK) are very potent and irreversible inhibitors of serine proteases. Availability depending on peptide sequence.

Ester (OMe/ OEt) : For structure-activity relationships (SAR), removal of charge, prodrug

p-Nitro-Aniline: a chromogen used as colorimetric enzyme substrate in many standard enzyme assays in cuvettes.Excitation maximum is 410 nm

Formylated peptide: The formyl peptide receptor (Fpr) family is well known for its contribution to immune defense against pathogens in human and rodent leukocytes.

Cholesterol conjugated peptide: the cholesterol-conjugated compounds are effective inhibitors of infectivity and membrane fusion. Cholesterol can be conjugated to a peptide via a N- or C-terminal inserted cysteine.

Cholesterol conjugated at peptide C-terminal:

 

Fatty acid conjugated peptides: Fatty acid conjugated peptides can be used for antibacterial activity or eukaryotic cell toxicity and so on. fatty acids like Caprylic acid (C8), Capric acid (C10), Lauric acid (C12), Myristic acid (C14), Palmitic acid (C16) or Stearic acid (C18) etc general conjugated on the peptide N-terminal or on the C-terminal via lysine side chain.

 

Linkers, Spacers used in the peptide synthesis

Depending upon design and application we can insert a spacer/linker between the tags, protein(KLH,BSA) Biotin, or fluorescent and the peptides. A common hydrophobic spacer is aminohexanoic acid (Ahx), and a common hydrophilic spacer is poly(ethylene) glycol (PEG).

Others please reference the below listed table: such as Gly, AEA, Ava, ANP Linker, Beta-Alanine, C12, GABA, etc...

 

What salt form would be preferred for custom peptide?

Custom peptides synthesized by SPPS are by default delivered as trifluoroacetic acid (TFA) salts. This is because TFA is used during the cleavage of the peptide from the solid support resin and the deprotection of amino acid side chains,Furthermore, TFA is commonly used as a component of the buffer system for HPLC purification of peptides. The TFA content typically ranges from 10% to 45%, depending on the number of Arg, Lys, and His residues in the sequence.

In some cellular assays, animal studies, and preclinical research cases, residual TFA may potentially cause the experiments to fail. Researchers can choose to remove the TFA or convert it to acetate or hydrochloride salts. Due to the additional TFA removal process, the cost will increases by approximately 20%. Biorunstar can provide TFA removal ( with TFA<10% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<1% guarantee) .

 

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